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  细胞健康检测金牌解决方案

Promega 细胞健康检测方案 

想您所想,为您提供更多!

Promega作为细胞健康检测方案的可靠供应商,产品覆盖了细胞活力检测、毒性检测、细胞凋亡检测、谷胱甘肽测定等多个方面。 

针对同一种细胞健康指标,我们提供多种方法,包括比色法、荧光法、生物发光法的检测方法,以满足您的不同需求。

  • Promega拥有的生物发光技术,可提供更灵敏,更快速的解决方案
  • 操作可简单至“加样-混合-检测”一步法,最快5-10分钟完成
  • 拥有多重检测技术,为单一细胞孔,提供更多检测指标数据
  • 丰富的产品线,总有一款适合您已有的检测设备
microscopy
检测类型 检测方法 目录号 检测参数/
生物标记
检测所
需时间
灵敏度 平板模式 仪器
细胞活力
CellTiter-Glo® Assay
G7570/G7571/
G7572/G7573
ATP 10 min 10 living cells 96/384/1536 Luminometer/CCD
  CellTiter-Glo® One Solution Assay
G8461/G8462 ATP 10 min 10 living cells 96/384/1536 Luminometer/CCD
  CellTiter-Glo® Assay
G6080/G6081/
G6082
Live-cell protease 0.5–3 h 40 living cells 96/384/1536 Fluorometer,AFC 
400Ex/505Em
  CellTiter-Blue® Assay
G8080/G8081/
G8082
Resazurin reduction by reducing equivalents 1–4 h 400 living cells 96/384/1536 Fluorometer,resorufin
560Ex/590Em
CellTiter 96® AQueous One Solution Assay
G3580/G3581/
G3582
MTS reduction by reducing equivalents 1–4 h 1,000 living cells 96/384 Spectrophotometer 
Abs 490 nm
  CellTiter 96® Non-Radioactive Cell Proliferation Assay (MTT)
G4000/G4100 MTT reduction by reducing equivalents 5h >1,000 living cells 96/384 Spectrophotometer 
Abs 570 nm
  BacTiter-Glo™ Assay
G8230/G8231/
G8232/G8233
ATP 5 min 10 living bacteria 96/384 Luminometer
细胞凋亡
  Caspase-Glo® 2 Assay
G0940/G0941 Caspase-2 activity 0.5 h Enzyme preparation 96/384/1536 Luminometer
Caspase-Glo® 8 Assay
G8200/G8201/
G8202
Caspase-8 activity 0.5 h ~1,000 apoptotic cells 96 Luminometer
Caspase-Glo® 9 Assay
G8210/G8211/
G8212
Caspase-9 activity 0.5 h ~1,500 apoptotic cells 96 Luminometer
Apo-ONE® Caspase 3/7 Assay
G7790/G7791/
G7792
Caspase-3/7 activity 1–18 h 625 apoptotic cells 96/384/1536 Fluorometer,R110,
499Ex/521Em
Caspase-Glo® 3/7 Assay
G8090/8091/
G8092/G8093
Caspase-3/7 activity 0.5 h 100 apoptotic cells 96/384/1536 Luminometer
  Caspase-Glo® 6 Assay
G0970/G0971 Caspase-6 activity 0.5 h Enzyme preparation 96/384/1536 Luminometer
  CaspACE™ Assay System, Colorimetric
G7351/G7220 caspase-3 activity 5h Enzyme preparation, cell extracts,tissue lysates 96 Spectrophotometer 
Abs 405nm
  CaspACE™ FITC-VAD-FMK In Situ Marker
G7461/G7462 caspase activity 1.5h In situ detection 96 florescence microscopy, 
flow cytometry
  DeadEnd™ Colorimetric TUNEL System
G7360/G7130 nuclear-DNA fragmentation   In situ detection  
DeadEnd™ Fluorometric TUNEL System
G3250 nuclear-DNA fragmentation   In situ detection at  single cell level or cell suspension     florescence microscopy, 
flow cytometry
细胞毒性
  CytoTox-Glo™ Assay
G9290/G9291/
G9292
Dead-cell protease release 15 min 10 dead cells 96/384/1536 Luminometer
  CytoTox-Fluor™ Assay
G9260/G9261/
G9262
Dead-cell protease release 0.5–3 h 10 dead cells 96/384 Fluorometer,R110 
485Ex/520Em
  CytoTox-ONE™ Homogeneous Membrane Integrity Assay
G7890/G7891/
G7892
LDH release 10 min 200 dead cells 96/384 Fluorometer,resorufin 560Ex/590Em
CytoTox 96® Non-Radioactive Cytotoxicity Assay
G1780 LDH release 30-60min   96 Spectrophotometer  
Abs 490 nm
CellTox™ Green Cytotoxicity Assay
G8741/G8742/
G8743/G8731
DNA binding by cell impermeable dye 15min 50 dead cells 96/384/1536 Fluorometer,
485Ex/520Em 
Proprietary dye
多重检测
  MultiTox-Glo Multiplex Cytotoxicity Assay
G9270/G9271/
G9272
Viability + cytotoxicity;live- + dead-cell protease 0.5 h 40 living cells,10 dead cells 96/384/1536 Fluorometer,AFC 
400Ex/505Em 
Luminometer
  MultiTox-Fluor Multiplex Cytotoxicity Assay
G9200/G9201/
G9202
Viability + cytotoxicity;live- + dead-cell protease 0.5–3 h 40 living cells,10 dead cells 96/384/1536 Fluorometer,AFC 
400Ex/505Em R110 
485Ex/520Em
  ApoLive-Glo™ Multiplex Assay
G6410/G6411 Viability + apoptosis;live-cell protease+ caspase-3/7 1–3 h ~40 living cells,100 apoptotic cells 96/384 Fluorometer,AFC 400Ex/505Em 
Luminometer
  ApoTox-Glo™ Triplex Assay
G6320/G6321 Viability, cytotoxicity + apoptosis live- + dead-cell protease +caspase-3/7 1–3 h ~40 living cells,100 apoptotic cells 96/384 Fluorometer,AFC 400Ex/505Em R110 485Ex/520Em 
Luminometer
  One-Glo™+ Tox Assay
E7110/E7120 Viability + reporter geneexpression;live-cell protease + luciferase activity 0.6–3 h ~40 living cells 96/384 Fluorometer,AFC 400Ex/505Em 
Luminometer
线粒体功能
  Mitochondrial ToxGlo™ Assay
G8000/G8001 Mitochondrial toxicity; deadcell protease + ATP;(Crabtree effect, galactose medium) 0.6–3 h   96/384 Fluorometer,R110 485Ex/520Em 
Luminometer
谷胱甘肽测定
  GSH-Glo™ Glutathione Assay
V6911/V6912 GSH 30 minutes   96/384 Luminometer
  GSH/GSSG-Glo™ Assay
V6611/V6612 GSH/GSSG 1 hour   96/384 Luminometer



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